Protein Sciences
Bringing the right protein to the table is often a critical first step in advancing a discovery program. Whether your team needs help designing constructs, troubleshooting expression challenges, or characterizing protein quality, I provide expertise to ensure you have the right reagents and insights to move forward with confidence.
Protein Engineering & Construct Design
Well-designed constructs can make the difference between a successful project and months of frustration. I help clients design and optimize DNA constructs tailored for yield, stability, and downstream applications.
Services include:
- Feasibility assessments – evaluate target tractability and recommend practical expression/purification strategies before committing major resources
- DNA construct design – optimize for expression, folding, and stability
- Tag incorporation – rational placement of purification, immobilization, or visualization tags (His, Strep, Avi, GFP, etc.) to support downstream workflows
- Fusion protein design – engineer multifunctional proteins by linking distinct domains, such as creating reporter biosensors, receptor chimeras with altered signaling or localization, or targeted fusions with antibody fragments.
- Structure- and sequence-guided design – apply structural insight, homology modeling, or sequence analysis to improve construct outcomes
Protein Expression & Purification
Access to pure, well-behaved protein is essential for both mechanistic studies and therapeutic development. I bring deep experience across various expression systems and purification strategies to deliver high-quality protein that is suitable for assays, structure determination, or therapeutic development.
Services include:
- Expression troubleshooting – resolve low expression, aggregation, or misfolding problems
- Screening approaches – use FSEC and small-scale trials to rapidly identify stable constructs and conditions
- Purification workflows – design purification strategies (utilizing affinity, size-exclusion, or ion exchange, etc. chromatography) to achieve purity and homogeneity
- Scale-up support – adapt workflows from analytical-scale to larger production runs for assays or structure determination
- Protein stability and formulation – optimize buffer design, additives, and storage conditions to extend shelf-life and reproducibility
- CRO guidance – recommend vendors, draft scopes of work, manage and troubleshoot outsourced expression/purification projects
Protein Analytics
A protein isn’t useful until you know it behaves as expected. I support clients in assessing protein quality, stability, and interactions to ensure reagents are fit-for-purpose and results can be trusted.
Services include:
- Oligomeric state and interaction mapping – confirm complex formation and/or identify binding partners
- Stability profiling – evaluate protein behavior under assay or storage conditions
- Readiness checks – assess homogeneity and stability to determine suitability for assays or structure determination before launching costly campaigns
Membrane Protein Expertise
Membrane proteins are notoriously difficult to work with, but central to many therapeutic programs (e.g., GPCRs, ion channels, and transporters). I provide specialized expertise in membrane protein expression, stabilization, and reconstitution – enabling studies that many groups cannot tackle in-house.
Services include:
- Expression and purification strategies – design workflows tailored to membrane proteins
- Detergent screening and stabilization – identify conditions that maintain functional, stable protein
- Nanodisc and proteoliposome reconstitution – prepare proteins in native-like environments for functional assays
Why Partner with Me?
With extensive experience across diverse protein classes and systems, I bridge the gap between concept and execution. Whether you need a single construct designed, or a complete workflow from gene to purified protein, I provide practical solutions tailored to your project’s goals.
Interested in working together? Contact me